We offer a full time PhD and/or postdoctoral research scientist position for the duration of three years.
The planed research project will focus on the role of Store-operated Calcium Entry (SOCE) in Skeletal Muscle (FWF project P31563), which serves an important (malfunction induces skeletal myopathy) yet unknown physiological role. SOCE occurs in a phasic manner during excitation-contraction coupling (Koenig 2018), and it appears that SOCE involves calcium nanodomains around STIM1 calcium sensors within the sarcoplasmic reticulum that are responsible for the gating of ORAI1 calcium channels in the t-tubular system (Koenig 2019). The current project aims to define the biophysical properties of SOCE and elucidate its physiological role in skeletal muscle. To address these questions we will employ confocal calcium imaging with fluorescent dyes in living single muscle fibres derived from rat, genetically modified mouse, and human muscle (biopsy-derived). - Koenig 2018, Biochim Biophys Acta Mol Cell Res. 1866(7):1239-1248. doi: 10.1016/j.bbamcr.2019.02.014. - Koenig 2019, Commun Biol. 1:31. doi: 10.1038/s42003-018-0033-7. We are looking for a PhD candidate or postdoctoral research scientist with a strong interest in muscle physiology, with good fine-motor skills, and biophysical comprehension. Excellent written and oral English language skills are essential, as well as high accuracy, reliability and good interpersonal skills. You will be part of a young team and will contribute your knowledge and skills to support the successful project. Innovative ideas are equally welcome as are independence in the design and performance of experiments. Methods applied in our group include: Calcium imaging, confocal microscopy, electrophysiology (patch clamp technique), skinned fibre preparation, isolation of ventricular cardiomyocytes (Langendorff), human iPSC- derived cardiomyocytes, genetically modified mice, cell culture, standard biochemistry and molecular biology. Previous experience with the listed methods in particular confocal microscopy and electrophysiology are highly advantageous. Please send your application including a CV, a motivation letter (PhD) or summary of current work (postdoc), and the names of at least two referees via email to Xaver König. The position will be available as of October 2019 in the group of DI Xaver König PhD at the Center for Physiology and Pharmacology, Dept. of Neurophysiology and –pharmacology, Medical University of Vienna. The salary will be in line with the standards of the Austrian Science Fund (FWF).